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KMID : 0545120070170020342
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Journal of Microbiology and Biotechnology
2007 Volume.17 No. 2 p.342 ~ p.347
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Characterization of Site-Specific Recombination by the Integrase MJ1 from Enterococcal Bacteriophage ΦFC1
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Park Mi-Ok
Lim Ki-Hong
Kim Tae-Hyoung
Chang Hyo-Ihl
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Abstract
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Bacteriophage ΦFC1 integrase (MJ1) was previously shown to perform a site-specific recombination between a phage attachment site (attP) and a host attachment site (attB) in its host, Enterococcus faecalis, and also in a non-host bacterium, Escherichia coli. Here, we investigated biochemical features of MJ1 integrase. First, MJ1 integrase could perform in vitro recombination between attP and attB in the absence of additional factors. Second, MJ1 integrase interacted with att sites. Electrophoretic mobility shift assays and DNase I footprinting revealed that MJ1 integrase could efficiently bind to all the att sites and that MJ1 integrase recognized relatively short sequences (~50 bp) containing an overlapping region within attB and attP. These results demonstrate that MJ1 integrase indeed catalyzes an integrative recombination between attP and attB, the mechanism of which might be simple and unidirectional, as found in serine integrases.
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KEYWORD
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attB, attP, Enterococcus faecalis, ΦFC1, integrase
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